Fig. 5.
Comparison of the effects of CFTRinh-172 on different CFTR orthologs. A: summary of mean % inhibition of hCFTR (n = 16), kfCFTR (n = 5), pCFTR (n = 4), and sCFTR (n = 12) with 5, 10, and 20 μM CFTRinh-172. The orthologs h-, kf-, and pCFTR were inhibited by CFTRinh-172, although pCFTR was much less responsive (20.6 ± 5.1% at 20 μM). However, sCFTR was insensitive to CFTRinh-172 at all concentrations (1.9 ± 0.9% inhibition at 5 μM, 4.9 ± 1.2% at 10 μM, and 8.5 ± 1.5% at 20 μM). The lack of inhibition of sCFTR and pCFTR by CFTRinh-172 is highly significant compared with hCFTR (P < 0.001 at all concentrations). Values are means ± SE. *P < 0.05; **P < 0.01; ***P < 0.001 compared with hCFTR. B and C: representative current-voltage (I-V) plots from oocytes expressing hCFTR (B) and sCFTR (C). FOK, forskolin.