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Doxorubicin stimulates atrogin-1/MAFbx mRNA and protein. A: data depict atrogin-1/muscle atrophy F-box (MAFbx) mRNA in myotubes 6, 16, 24, and 48 h after doxorubicin exposure (n = 6/group at all time points). B: averaged data for atrogin-1/MAFbx protein 24 and 48 h following doxorubicin exposure (n = 13 for vehicle and 24 h; n = 4 for 48 h). Data are means ± SE. *P < 0.05 vs. control. Representative Western blots may not be from contiguous lanes. AU, arbitrary units.
