Figure 4:
Uniplex second PCR products carried out with the specific primers for Val96Ala, Leu141Leu, Val154Ile, Pro310Ser, Ser311Cys, TaqI A, −141C Ins/Del, and A-241G polymorphisms. The genotypes of the samples are homozygous wild-type for Val96Ala, Leu141Leu, Val154Ile, Pro310Ser, Ser311Cys, and −141C Ins/Del polymorphisms, and heterozygotes for TaqI A and A-241G polymorphisms. M: 100-bp DNA ladder; Wt: wild-type; Mt: mutant-type.