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. 2011 Oct-Dec;18(4):44–57.

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© Penerbit Universiti Sains Malaysia, 2011

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Figure 5: — Optimisation of the allele-specific multiplex PCR. Agarose gel electrophoresis of the products from the second PCR showing the effect of different MgCl₂ and Taq polymerase concentrations. (A) Results of the second PCR products amplified at concentration of MgCl₂ 2.0 mM and Taq polymerase 1.0 U showing the presence of non-specific bands in all PCR sets. (B) Second PCR products amplified at a concentration of MgCl₂ 1.0 mM and Taq polymerase 0.5 U. Results showed that MgCl₂ and Taq polymerase concentrations have a significant effect on elimination of the non-specific bands, and all the expected bands were visualised clearly. M: 100-bp DNA ladder; Wt: wild-type; Mt: mutant-type.

Figure 5: — Optimisation of the allele-specific multiplex PCR. Agarose gel electrophoresis of the products from the second PCR showing the effect of different MgCl₂ and Taq polymerase concentrations. (A) Results of the second PCR products amplified at concentration of MgCl₂ 2.0 mM and Taq polymerase 1.0 U showing the presence of non-specific bands in all PCR sets. (B) Second PCR products amplified at a concentration of MgCl₂ 1.0 mM and Taq polymerase 0.5 U. Results showed that MgCl₂ and Taq polymerase concentrations have a significant effect on elimination of the non-specific bands, and all the expected bands were visualised clearly. M: 100-bp DNA ladder; Wt: wild-type; Mt: mutant-type.