Figure 2.

Flow cytometric single cell sorting. PAEC suspended in EGM-2 were sorted into 96-well plates by FACS. FSC-A/SSC-A dot plot was used to define gate for endothelial cells. In order to prevent the sorting of >1 endothelial cell in each well aggregate correction was performed. Aggregates lead to increased SSC-W and FSC-W due to prolonged scatter of the laser due to their increased size. Use of aggregate exclusion led to 97% of wells receiving a single endothelial cell that was able to adhere to the collagen matrix when assessed 24 hrs. after sorting.