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. 2001 May 1;98(10):5786–5791. doi: 10.1073/pnas.101116298

Figure 1.

Figure 1

Inh I or Inh II suppresses UVB-induced AP-1 activity in a concentration-dependent manner. Stably transfected JB6 P+1-1 AP-1 luciferase reporter cells were cultured and starved as indicated in the text. After a 30-min treatment of the cells with Inh I or Inh II at the concentrations indicated, the cells were or were not exposed to UVB irradiation (4 kJ/m2) and cultured for another 12 h before harvest and determination of AP-1 activity. AP-1 activity is expressed as relative luciferase units as assessed by a luminometer. (A) Inh I or Inh II treatment resulted in slight, but insignificant (P > 0.05; mean ± SD of triplicate experiments, six wells each), increase of basal AP-1 activity. (B) UVB stimulation alone induced a 13.8- to 14.3-fold increase in AP-1 activity. Inh I (40–80 μM) or Inh II (20–80 μM) significantly inhibited AP-1 activity in response to UVB. ∗, P < 0.05; mean ± SD for triplicate experiments, six wells each.