Figure 2.

Resveratrol dose-dependent inhibition of carcinogen-induced COX-2 protein and gene expression. (A) HBMEC were serum-starved in the presence of various concentrations of resveratrol and in combination with vehicle or 1 μM PMA for 18 hours. Lysates were isolated, electrophoresed via SDS-PAGE, and immunodetection of COX-2 and GAPDH performed as described in the Methods section (NS, non specific immunoreactivity). (B) Scanning densitometry of COX-2 expression was only performed in PMA-treated cells since no COX-2 was detectable in vehicle-treated HBMEC. Densitometric data of a representative blot is shown out of three independent experiments. (C) Total RNA isolation, RT-PCR, and qPCR were performed as described in the Methods section to assess COX-2 gene expression in the above-described conditions. (PMA = 1 μM; Resveratrol = 30 μM).

Notes: Data are representative of three independent qPCR experiments. Probability values of less than 0.05 were considered significant, and an asterisk (*) identifies such significance to the respective PMA treatment.