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. 2001 May 1;98(10):5798–5803. doi: 10.1073/pnas.091479298

Figure 4.

Figure 4

(A) Mitf regulates endogenous cathepsin K mRNA expression in human osteoclasts. Primary human osteoclasts cultured from bone marrow were infected at day 11 with adenoviruses encoding wild-type Mitf, GFP, and mutant Mitf followed by harvesting total RNA after 46 and 67 h. Northern blots were probed for cathepsin K, GAPDH, and c-fms. Comparable overexpression of wild-type and mutant Mitf proteins was verified by Western blot (Bottom) with anti-Mitf antibody. (B) TRAP mRNA levels also increase upon overexpression of wild-type Mitf. Day 14 and day 11 primary human osteoclasts were infected with adenoviruses encoding wild-type Mitf, GFP, and mutant Mitf followed by harvesting total RNA after 67 and 46 h, respectively.