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Quantitative RT-PCR analysis of genes associated with P distribution. Wild-type (WT), pht1;5–1 mutant, and Pht1;5-overexpression (Pht1;5-Oe) seedlings were grown hydroponically for three weeks in high-Pi (1.25 mM Pi) media. Total RNA isolated from these plants was used for qRT-PCR. At4g26410 was used as an internal reference gene, and the values, normalized to WT levels, are the means +/− SE of two independent biological replicates run in triplicate.
