Figure 1.

Subcellular localization of GFP-AtROPGAP1 in epidermal cells of Arabidopsis. Arabidopsis epidermal cells transiently transformed with GFP-ROPGAP1 (green, first row) alone or together with CA AtROP6, CA AtROP4 or DN AtROP6 expression constructs. Soluble RFP (red, second row) was co-transformed to label the cytoplasm and nucleoplasm GFP-AtROPGAP1 alone or co-expressed with unlabeled DN AtROP6 displays cytoplasmic localization, whereas co-expression of unlabeled, membrane-associated CA AtROP4 or CA AtROP6 results in recruitment of GFP-AtROPGAP1 to the plasma membrane. Pictures are maximum projections of 20–30 optical sections at 2 μm increments. Scale bars represent 20 μm. In merged pictures signal co-localization is represented by yellow color (third row). Similar results are obtained for GFP-AtROPGAP4 (online Supplement of Hoefle et al.¹³).