Figure 2.

Fungal development on Atropgap4 and on wild type at 48 h after inoculation. Each 10 Arabidopsis rosette leaves of Atropga4 (SALK_038694) and wild type AtROPGAP4 (Col-0) have been inoculation with conidia of E. cruciferarum and fixed for microscopy 48 h later according to Hoefle et al.¹³ Analysis of 150–200 individual interaction sites on each leaf revealed differences in the fungal success to form a haustorium in the first attacked epidermal pavement cell and to develop more than 3 epiphytic hyphae per spore at this time of the interaction. ** indicates significant differences between wild type and Atropgap4 at p < 0.01 (unpaired Student’s t-test). Repetition of the experiment led to very similar results.