Figure 4.

Nematode susceptibility assays of the atprx53 mutant and three AtPRX53OE lines. (A) The atprx53 mutant showed a consistent trend of increased susceptibility to Heterodera schachtii in four experiments (Exp) with Exp 1 showing statistically significant effects. (B) Two out of three AtPRX53OE lines showed significantly reduced susceptibility to H. schachtii. Arabidopsis plants were planted into 12-well plates containing modified Knop’s medium, and ten-day-old plants were inoculated with ~200 surface-sterilized H. schachtii second-stage juveniles per plant. Three weeks after inoculation, the number of fourth-stage (J4) females per plant was determined. Mean values significantly different from the corresponding wild type were determined by modified t-test (p < 0.05).