Figure 1.

Wounding and abiotic stress cause ATP accumulation around A. thaliana roots.A. thaliana (Col-0) was grown for seven days as described previously on MS medium with 1% (w/v) sucrose.¹³ Eight roots of similar lengths were placed in the well of a 96-well plate containing sterile liquid MS/1% (w/v) sucrose, with or without test substances. ATP content of samples was determined with a luciferin/luciferase assay (Molecular Probes), using a Fluostar Optima plate reading luminometer. Wounding was inflicted by moving a gloved finger up and down the roots to damage root hairs prior to immersion in assay medium. Results are mean ± SEM from 3 independent trials.