TABLE 1.

Simultaneous quantification of 30 acylcarnitines of palmitate oxidation.

	Acylcarnitine	m/z	Mean pmol	SEM pmol	LOD pmol	LOQ pmol
High abundance	C2:0	260.2	2097.70	66.61	27.24	57.14
	C12:0	400.3	462.82	41.83	3.48	9.91
	C16:0	456.4	381.65	7.42	0.86	2.30
	C6:0	316.3	137.08	6.12	10.61	26.26
	C10:0	372.3	136.51	8.28	2.90	7.73
	C8:0	344.3	113.34	6.38	6.50	17.50
	C14:0	428.4	43.58	0.82	0.41	1.13
	C16:0-OH	472.4	11.31	0.39	0.60	1.25
	C16:1	454.4	11.09	0.21	0.12	0.34
	C14:1	426.4	3.96	0.13	0.19	0.60
	C14:0-OH	444.4	3.50	0.12	0.24	0.65
	C18:1	482.4	1.67	0.06	0.07	0.19
Medium abundance	C4:0	288.2	79.94	3.25	16.68	38.30
	C10:1	370.3	23.82	2.60	4.66	12.43
	C5:0-DC	388.3	8.78	0.81	1.58	4.30
	C18:0	484.4	3.05	0.11	0.36	0.94
	C16:1-OH	470.4	1.31	0.06	0.17	0.48
	C12:1	398.3	0.93	0.07	0.12	0.31
	C18:2	480.4	0.63	0.04	0.07	0.19
	C18:1-OH	498.4	0.23	0.02	0.05	0.14
Low abundance	C6:0-DC	402.3	34.31	4.14	17.64	41.57
	C3:0	274.2	20.63	1.92	15.31	37.54
	C4:0-DC	374.3	4.41	0.38	2.90	7.34
	C5:1	300.2	3.71	0.37	2.33	4.68
	C8:1	342.3	3.20	0.33	1.63	4.18
	C12:0-DC	486.4	0.33	0.02	0.22	0.50
	C18:0-OH	500.4	0.13	0.01	0.05	0.14
	C16:2	452.4	0.07	0.01	0.05	0.14
	C16:2-DC	476.5	0.05	0.01	0.02	0.10
	C16:1-DC	540.4	0.03	0.01	0.02	0.10
Below detection limit	C3:0-DC	360.2	11.02	1.16	12.48	32.14
	C5:0	302.2	9.68	0.48	20.11	45.34
	C6:0-OH	332.3	7.71	1.19	7.99	23.21
	C4:0-OH	304.2	2.84	0.35	3.48	9.22
	C5:0-OH	318.2	2.77	0.23	4.85	12.70
	C10:2	368.3	0.51	0.08	0.79	1.99
	C14:2	424.3	0.25	0.03	0.29	0.77
	C12:2	396.3	0.13	0.01	0.19	0.46
	C18:2-OH	496.4	0.10	0.01	0.31	0.74
	C18:1-DC	568.5	0.04	0.01	0.05	0.12
	C16:0-DC	542.4	0.02	0.004	0.12	0.38
	C18:0-DC	570.5	0.01	0.004	0.07	0.19
	C18:2-DC	568.5	0.01	0.002	0.05	0.17

Metabolites were categorized according to their abundance (pmol) following determination by ESI-MS/MS, prior to normalization to citrate synthase activity (n = 11 samples of within-run analyses).