TABLE 1.
Plasma lipids, liver lipid composition, and biliary excretion of sterols in wild-type and SR-BI knockout mice in response to hepatic apolipoprotein E overexpression
| Wild-type | SR-BI knockout | |||
| AdNull | AdhApoE3 | AdNull | AdhApoE3 | |
| Plasma | ||||
| Total cholesterol (mg/dl) | 69.5 ± 1.9 | 65.5 ± 2.6 | 147.6 ± 9.5 | 140.5 ± 12.1 |
| Free cholesterol (mg/dl) | 29.0 ± 0.7 | 31.3 ± 0.9 | 86.4 ± 4.9 | 81.3 ± 7.8 |
| Esterified cholesterol (mg/dl) | 40.5 ± 1.6 | 34.3 ± 3.1 | 61.1 ± 6.4 | 59.2 ± 5.4 |
| Phospholipids (mg/dl) | 178.6 ± 12.7 | 148.6 ± 8.0 | 191.8 ± 10.8 | 214.7 ± 16.5 |
| Triglycerides (mg/dl) | 82.9 ± 9.2 | 80.0 ± 8.9 | 59.8 ± 6.9 | 79.9 ± 10.8 |
| Liver | ||||
| Total cholesterol (nmol/mg liver) | 6.3 ± 0.5 | 7.8 ± 0.4a | 8.5 ± 0.3 | 8.3 ± 0.4 |
| Free cholesterol (nmol/mg liver) | 5.4 ± 0.4 | 5.9 ± 0.3 | 6.7 ± 0.1 | 6.4 ± 0.1 |
| Esterified cholesterol (nmol/mg liver) | 0.8 ± 0.2 | 2.0 ± 0.1a | 1.7 ± 0.3 | 2.0 ± 0.3 |
| Phospholipids (nmol/mg liver) | 28.2 ± 1.5 | 25.8 ± 0.9 | 31.9 ± 0.7 | 29.5 ± 0.6a |
| Triglycerides (nmol/mg liver) | 12.8 ± 3.1 | 58.3 ± 5.0a | 22.2 ± 1.6 | 32.8 ± 4.5 |
| Bile | ||||
| Bile flow (μl/min/100 g bw) | 10.3 ± 0.6 | 10.2 ± 0.2 | ND | ND |
| Biliary bile acid secretion (nmol/min/100 g bw) | 866 ± 86 | 762 ± 45 | ND | ND |
| Biliary phospholipid secretion (nmol/min/100 g bw) | 61.6 ± 5.0 | 82.6 ± 6.8a | ND | ND |
| Biliary cholesterol secretion (nmol/min/100 g bw) | 3.9 ± 0.2 | 4.0 ± 0.3 | ND | ND |
On day 4 after adenovirus injection, bile was collected continuously for 30 min, plasma samples were taken, and livers were harvested and snap-frozen in liquid nitrogen. Plasma lipids, liver lipids, and biliary output rates of bile acids, phospholipids, and cholesterol were determined as described in Materials and Methods. Values are means ± SEM; n = 5–8 mice for each condition. AdhApoE3, recombinant adenovirus expressing human apoE3; AdNull, empty control adenovirus; SR-BI, scavenger receptor class B type I; bw, body weight; ND, not determined.
Significantly different from the respective AdNull-injected controls as assessed by Mann-Whitney U-test (at least P < 0.05).