TABLE 2.
Hepatic mRNA expression in wild-type mice in response to hepatic apolipoprotein E overexpression
| Wild-type | ||
| AdNull | AdhApoE3 | |
| Sr-b1 | 1.00 ± 0.03 | 0.70 ± 0.03a |
| Abcb11 | 1.00 ± 0.06 | 0.60 ± 0.04a |
| Abcb4 | 1.00 ± 0.07 | 0.91 ± 0.05 |
| Abcg5 | 1.00 ± 0.08 | 0.62 ± 0.04a |
| Abcg8 | 1.00 ± 0.06 | 0.69 ± 0.06a |
| Cyp7a1 | 1.00 ± 0.23 | 0.91 ± 0.13 |
| Cyp27a1 | 1.00 ± 0.09 | 0.46 ± 0.04a |
| Cyp8b1 | 1.00 ± 0.07 | 0.74 ± 0.02a |
| Srebp2 | 1.00 ± 0.10 | 0.70 ± 0.02a |
| Ldlr | 1.00 ± 0.09 | 0.71 ± 0.04 |
| Hmgcr | 1.00 ± 0.16 | 0.74 ± 0.09 |
| Abca1 | 1.00 ± 0.02 | 0.92 ± 0.06 |
Livers of mice administered the respective adenoviruses were harvested on day 4 after adenovirus injection and snap-frozen in liquid nitrogen. mRNA expression levels were determined by real-time quantitative PCR as described in Materials and Methods. Values are means ± SEM; n = 6 mice for each condition. Within each set of experiments, gene expression levels are related to the respective AdNull-injected controls. AdhApoE3, recombinant adenovirus expressing human apoE3; AdNull, empty control adenovirus.
a
Significantly different from the respective AdNull-injected controls as assessed by Mann-Whitney U-test (at least P < 0.05).