Fig. 5.

Niacin-stimulated ABCA1 transcription is dependent on DR4. A: RT-qPCR. HepG2 cells were incubated with niacin for 48 h and total RNA were extracted for RT-qPCR as described in Materials and Methods. * P < 0.01. B: Promoter assay. pGL3-ABCA1 (WT) or pGL3-ABCA1 containing mutated DR4 (MUT) were transfected into HepG2 cells for dual luciferase assay in the presence or absence of 0.5 mM niacin (N) and 10 μ M 22-(R) hydroxycholesterol (HC). Data are expressed as mean ± SD of three experiments; *P < 0.01.