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. 2012 May;53(5):951–963. doi: 10.1194/jlr.M021154

Fig. 3.

Fig. 3.

Recombinant FVB ApoA-I and helix 9/10 synthetic peptide clear DMPC multilamellar vesicles more rapidly than C57 ApoA-I and 9/10 peptide, with FVB-ApoA-I forming larger HDL-sized particles. A: Multilamellar vesicles of DMPC (final concentration, 0.25 mg/ml) were added to 0–0.2 mg/ml C57 or FVB T7-ApoA-I or ApoA-I-9/10 synthetic peptide, and the absorbance at 490 nm was followed over the course of 1 h and normalized to the initial absorbance reading. Representative curves at 0.05 mg/ml are shown (minimum of five samples each). Percent turbidity of DMPC remaining at 10 min was plotted against A-I or 9/10 peptide concentration and fit to Michaelis-Menten kinetics. * P < 0.05 compared with C57. # P < 0.05 compared with corresponding T7-ApoA-I. B: Nondenaturing PAGE gel of 0.08 mg/ml T7-ApoA-I-cleared DMPC vesicles after 1 h clearance.