Fig. 6.
The differences in C57 or FVB apolipoprotein-mediated efflux of cholesterol from J774 macrophages are dependent on the type of acceptor. J774 macrophages were loaded with acetylated-LDL and labeled with 3H-cholesterol in the presence of an ACAT inhibitor and CPT-cAMP as described in Materials and Methods. Efflux of 3H-cholesterol to varying concentrations of C57 or FVB (A) recombinant T7-ApoA-I, (B) synthetic ApoA-I helix 9/10 peptide, or (C) HDL were measured. Data points show the mean ± SD of triplicate samples.