Fig. 9.

HDL secreted from FVB hepatocytes are no more dense and do not possess more cholesterol or phospholipids than HDL secreted from C57 hepatocytes. Primary hepatocytes were isolated from mature female C57 or FVB mice as described in Materials and Methods. After loading with (A) ³H-cholesterol or (B) ³H-choline cells were washed and incubated for 24 h. Media was harvested and separated into lipoprotein fractions by density gradient ultracentrifugation. Data points show the mean ± SD of triplicate samples.