Fig. 1.

CEC of sera from subjects before, immediately after, and two days after LDL-apheresis. SR-BI-mediated CEC (A) and ABCG1-mediated CEC (B). Efflux of radiolabeled cholesterol to 2% (v/v) serum was measured as described in Methods. Specifically, SR-BI-mediated CEC was measured in Fu5AH hepatoma cells (A) and ABCG1-mediated CEC was the difference in efflux between hABCG1 overexpressing CHO and control CHO cells (B). Each point represents the average efflux to serum from single patients harvested before LDL-apheresis (open circles), immediately after LDL-apheresis (filled squares), and two days after LDL-apheresis (open triangles) (n = 26). Statistical significance was estimated by paired, two-tailed t-tests; ***P < 0.001.