Fig. 2.

CEC of sera from subjects before, immediately after, and two days after LDL-apheresis. AD-mediated CEC (A) and ABCA1-mediated CEC (B). Efflux of radiolabeled cholesterol to 2% (v/v) serum was measured as described in Methods. Specifically, AD-mediated CEC was measured in J774 macrophages in basal conditions (A), and ABCA1-mediated CEC was the difference in efflux between cAMP-treated J774 and J774 in basal conditions (B). Each point represents the average efflux to serum from single patients harvested before LDL-apheresis (open circles), immediately after LDL-apheresis (filled squares), and two days after LDL-apheresis (open triangles) (n = 23). Statistical significance was estimated by paired, two-tailed t-tests; *P < 0.05; **P < 0.01; ***P < 0.001.