Figure 2. Genomic alignment of 454 reads with the published thylacine mtDNA genome.

The 454 generated sequences produced 16 individual alignments with the published genome. Eleven of these fragments covered seven regions and were nearly identical (only two base differences over 2.24 kilobases). The other five fragments covered three locations (ATP6, COX1 and 16s rRNA genes) and were highly divergent from previously published sequences. Therefore, these sequences likely represent NuMts. Numbers above/below fragments indicate the nucleotide similarity to the published thylacine mtDNA genome and total number of polymorphisms in brackets.