Table 1. List of major antibodies and cocktail configurations used for MHC staining of rat, mouse, and human skeletal muscle.
| Species | Primary Antibody Cocktails and Concentrations | MHC Reactivity | Secondary Antibody Cocktails and Concentrations |
| Rat and Mouse | BA-F8 (1∶50) | I | Alexa Fluor 350 IgG2b 1∶500 (blue) |
| SC-71 (1∶600) | IIa | Alexa Fluor 488 IgG1 1∶500 (green) | |
| BF-F3 (1∶100) | IIb | Alexa Fluor 555 IgM 1∶500 (red) | |
| Rat and Mouse | SC-71 (1∶600) | IIa | Alexa Fluor 488 IgG1 1∶500 (green) |
| 6H1 (1∶50) | IIx | Alexa Fluor 555 IgM 1∶500 (red)1 | |
| Human | BA-F8 (1∶50) | I | Alexa Fluor 350 IgG2b 1∶500 (blue) |
| SC-71 (1∶600) | IIa+IIx* | Alexa Fluor 488 IgG1 1∶500 (green) | |
| 6H1 (1∶50) | IIx | Alexa Fluor 555 IgM 1∶500 (red) | |
| Human | BA-F8 (1∶50) | I | Alexa Fluor 350 IgG2b 1∶500 (blue) |
| BF-35 (1∶100) | I+IIa | Alexa Fluor 488 IgG1 1∶500 (green) | |
| 6H1 (1∶50) | IIx | Alexa Fluor 555 IgM 1∶500 (red) |
All antibody cocktails are prepared in block solution (10% goat serum in PBS).
*
Non-specific cross-reactivity with MHCIIx was observed in human skeletal muscle.
1
Fibers positive for MHCIIx showed red staining, but for presentation and discussion purposes in rats and mice these fibers were pseudo-colored purple to avoid confusion with MHCIIb positive fibers.