Figure 5. The pGT insert in BG00076 decreases Skip expression level.

Quantitative real-time PCR analysis of Skip expression in whole adult flies. The amount of Skip mRNA from CS5 and BG00076 flies was normalized to cyclin K and is indicated in arbitrary units. Values are shown as the mean ± the SEM and are averaged from four separate biological experiments (each replicated in quadruplicate). There is an approximately five fold decrease of Skip transcription in line BG00076. *** p<0.001 t-test.