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| Time (min) | Methanol (%) | Acetonitrile (%) | 0.1% formic acid (%) | Flow rate (µl/min) |
| 0 | 30 | 38 | 32 | 750 |
| 7.5 | 30 | 38 | 32 | 750 |
| 8.0 | 99.5 | 0.5 | 700 | |
| 10.0 | 99.5 | 0.5 | 700 | |
| 10.5 | 5 | 85 | 10 | 800 |
| 14.4 | 5 | 85 | 10 | 800 |
| 14.8 | 30 | 38 | 32 | 750 |
| 20.0 | 30 | 38 | 32 | 750 |
