Table 1. Summary of beta-cell-like phenotypes in FACS-sorted HIT-T15 cells.
| Green Cells (GFP+) | Red Cells (mCherry+) | |
| Ca2+ current densitya | Larger | Smaller |
| Extent of exocytosisb | Larger | Smaller |
| Glucose-stimulated increase in [Ca2+]i | Yes | No |
| Insulin gene transcription | Yes (high level) | Yes (low level) |
| Glucagon gene transcription | Yes (low level) | Yes (High level) |
a
Ca2+ current density is measured in pA/pF (current divided by capacitance), a measure of the flow of calcium in the membrane area. GFP+ cells showed 12.6±1 pA/pF, while mCherry+ cells showed 5±0.61 pA/pF (p<0.0001).
b
The percentage of cells exhibiting capacitance increase was 74% for GFP+ cells and 28% for mCherry+ cells.