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. 2012 Apr 18;7(4):e33502. doi: 10.1371/journal.pone.0033502

Figure 3. The nucleotidic divergence of HIV-1 RNA fragments correlates with their ability to stimulate IFN-α/β.

Figure 3

A) The black line shows the nucleotide divergence of HIV-1 compared to human genome, as measured by a Chi-square distance in a 500 bp sliding window along the HIV-1 hxb2 genome. The individual contributions of A, C, G, and U nucleotides to this divergence are shown respectively in red, blue, yellow and green. The HIV-1 genome map is schematically represented above the figure. B) Histogram represents the ISRE reporter activity as the ratio of firefly luciferase activity to control renilla luciferase activity determined in HEK 293 T cells cotransfected with HIV-1 RNA fragments and pISRE-Luc reporter. C) Correlation between the nucleotide divergence (x-axis) and the luciferase activation (y-axis) of the 38 RNA fragments from HIV-1 hxb2 genome. Each point corresponds to the average of four replicates and the correlation coefficient was computed only on these averages to not artefactually lower the p-value.