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. 2012 Apr 18;7(4):e34432. doi: 10.1371/journal.pone.0034432

Figure 5. Regulation of HER1 Y1068 or Y1173 phosphorylation downstream of G-proteins requires calmodulin/calcineurin activity.

Figure 5

(A) and (B) Time-course. HeLa cells were stimulated as indicated in Figure 3. Treatment with W-13 (a calmodulin inhibitor) or CsA (a calcineurin inhibitor) restored HB-EGF-dependent HER1 phosphorylation at Y1068, whereas KN-62 (a CaMKII inhibitor) had no effect. (C) Following prestimulation with [N33A]CXCL12 the HB-EGF-dependent HER1 phosphorylation at Y1068 and Y1173 was abolished at the plateau (15 minutes) and restored by W-13 or CsA; KN-62 had no effect. The means ±SD of 10 experiments are depicted.