Figure 1.sr45–1 de novo DNA methylation phenotype. (A) Flowering time of Columbia, sr45–1, dcl3–1 and sr45–1, dcl3–1 double before and after FWA transformation. Flowering time is measured as the total number of leaves at the time of flowering. (B) Methylation levels at endogenous and transgenic FWA after FWA transformation. The 594 base-pair repeated region in the 5′ UTR was analyzed. The methylation state of the FWA endogene should remain unaltered by the presence of the FWA transgene. All samples were analyzed in the T1 generation.