Analysis of cardiac function by noninvasive echocardiography in
conscious mice. (A) Transthoracic M-mode
echocardiographic tracings in 7-week-old wild-type
(Left), CSQ (Center), and
CSQ/βARKct (Right) mice. LV dimensions are
indicated with the double-sided arrows. EDD, end diastolic dimension;
ESD, end systolic dimension. Wild-type mice have normal chamber size,
whereas the CSQ mice have chamber dilation and depressed cardiac
function. The CSQ/βARKct mice have only moderate chamber
dilation and slightly reduced cardiac function as compared with the
wild-type mice. (B) Echocardiographic findings in
7-week-old wild-type and transgenic mice. LV EDD (Left)
and percent fractional shortening (Right) are shown.
Wild type, n = 15; βARKct, n
= 23; CSQ, n = 14; CSQ/βARKct,
n = 31. *, P < 0.0001,
CSQ vs. wild type; †, P < 0.0001,
CSQ/βARKct vs. CSQ; ‡, P < 0.0001,
CSQ/βARKct vs. wild type. (C) Data from
serial echocardiograms in the same mouse at 7 weeks (#1) and 11 weeks
(#2) of age for LV EDD. (D) Percent fractional
shortening in the same mouse. Wild type, n = 14;
βARKct, n = 22; CSQ, n = 3;
CSQ/βARKct, n = 28. *,
P < 0.001, CSQ/βARKct (#2) vs.
CSQ/βARKct (#1).