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. 2001 Apr 17;98(10):5815–5820. doi: 10.1073/pnas.081612998

Figure 4.

Figure 4

Effect of cholesterol depletion on the distribution of the α-secretase ADAM 10 in detergent-insoluble lipid rafts and in caveolae. (A) Localization of ADAM 10 in lipid rafts. HEK ADAM 10 cells with or without treatment for 30 min with 10 mM MβCD were extracted with 2% Triton X-100. After flotation in an OptiPrep step-gradient, the fractions were TCA precipitated and analyzed by Western blot with antihemagglutinin antibody for ADAM 10 detection. (B) HEK ADAM 10 cells were incubated for 4 h with filipin (1 μg/ml). Then the cell medium was collected and the secreted APPsα was quantified as described above. Statistical significance between control cells and filipin-treated cells was determined by Student's unpaired t test (*, P < 0.05).