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. 2001 Apr 17;98(10):5815–5820. doi: 10.1073/pnas.081612998

Figure 6.

Figure 6

Influence of lovastatin on activity and expression of the α-secretase ADAM 10 in HEK ADAM 10 cells. (A) Reversal of the lovastatin effect on APPsα secretion in HEK ADAM 10 cells by cholesterol. All cells were cultured in lipid-deficient medium either in the absence (lane 1) or in the presence of 0.3 mM cholesterol–MβCD complex (lane 2), in the presence of 1 μM lovastatin (lane 3), or in the presence of 1 μM lovastatin and 0.3 mM cholesterol–MβCD complex (lane 4). The medium was analyzed for secreted APPsα, as described in Fig. 1. Statistical significance between control cells and treated cells was determined by Student's unpaired t test (*, P < 0.01; **, P < 0.005)). (B) Membranes (200 μg of each) prepared from cells cultured in lipid-deficient serum in the absence (lane 1) or in the presence of 1 μM lovastatin were separated by electrophoresis and transferred to PVDF membranes. For detection of ADAM 10, membranes were probed with an antihemagglutinin antibody (Y-11).