FIGURE 2. Collagen binding to recombinant α2β1-Fc integrins.

Culture supernatants from CHO L761h cells. transfected with FLα2 and FLβ1 constructs (FLα2β1-Fc), TRα2 and TRβ1 constructs (TRα2β1-Fc) or no DNA (mock) were analysed by Fc-capture assays. Binding of collagen I (1 μg/ml) to captured proteins was measured in 5 mM Mg²⁺ (A) or 1 mM Mn²⁺ (B) in the absence (open bars) or presence of the activating anti-β1 mAb TS2/16 (diagonally-hatched bars) or 5 mM EDTA (cross-hatched bars). Supernatants from cells transfected with either α2 or β1 constructs alone did not show any collagen binding above that of supernatants from mock-transfected cells (data not shown). Absorbance values and error bars represent the mean and standard deviation of three replicate wells from one experiment, representative of four separate experiments.