Table I.

Summary of mAb reactivity with α2β1 integrin variants. CHO L761h cells were transfected with α2^1-1129-Fc (FL), α2^1-806-Fc (TR) or α2^1-806[I332G] (TRα2[I332G]), and β1^1-708 (FL), wild-type β1^1-455 (TR) or mutated β1^1-455. Recombinant receptors were captured from cell culture supernatants with goat anti-human Fc antibody onto microtiter plates and were analysed for reactivity with 5μg/ml anti-α2 mAbs (JA218, 12F1, P1E6 or Gi9), or anti-β1 mAbs (12G10, TS2/16, 8E3, 4B4, 15/7 or HUTS-4) by ELISA. Results are expressed as a percentage of wild type full-length or truncated α2β1-Fc binding, normalised to the binding of anti-α2 JA128 mAb. Results shown are from one experiment, representative of at least three separate experiments.

A
	JA218	12F1	P1E6	Gi9	12G10	TS2/16	8E3	4B4	15/7	HUTS4
FL α2β1	107.96	53.39	109.63	77.19	115.89	116.97	57.16	50.53	83.18	86.42
TRα2β1	100.00	54.25	100.30	67.90	101.05	108.20	55.80	50.25	45.10	65.25
TRα2β1[D130A]	89.21	43.18	89.43	51.16	97.86	98.17	52.41	42.28	55.53	73.64
TRα2β1[D137A]	103.10	54.65	128.62	66.71	101.51	110.32	61.81	49.44	24.02	51.66
TRα2β1[D138A]	100.73	57.87	112.22	71.67	98.21	107.11	60.19	65.48	2.42	24.88
TRα2β1[D226A]	95.77	56.08	101.76	69.63	103.48	98.55	62.73	53.35	55.26	75.04
TRα2β1[E169A]	98.92	59.35	106.93	64.99	109.41	110.94	58.86	64.30	60.34	91.35
TRα2β1[E169D]	99.18	56.33	101.41	67.49	103.59	109.94	49.39	51.37	71.65	88.86

B
	JA218	12F1	P1E6	Gi9	12G10	TS2/16	8E3	4B4	15/7	HUTS4
TRα2β1	100.00	83.15	102.00	88.75	68.30	64.65	63.20	52.55	93.15	81.90
TRα2[I332G] β1	91.72	91.72	97.87	92.32	77.09	72.37	67.74	66.82	91.77	85.62
TRα2[I332G] β1[D130A]	93.82	83.17	96.49	84.44	63.70	64.88	71.87	66.42	90.21	85.80
TRα2[I332G] β1[D137A]	130.62	96.40	97.31	89.80	75.04	77.79	100.19	77.98	88.11	83.53
TRα2[I332G] β1[D138A]	108.93	88.83	88.50	85.78	76.79	82.95	85.29	80.93	88.99	85.13
TRα2[I332G] β1[D226A]	112.55	87.56	90.83	93.59	80.81	84.75	109.85	89.59	94.04	93.92
TRα2[I332G] β1[E169A]	96.69	93.06	98.29	95.09	83.59	79.91	93.11	85.96	94.37	94.37
TRα2[I332G] β1[E169D]	118.06	109.45	92.50	112.63	84.06	83.41	108.62	95.99	105.67	100.06