Table II.

Cation-binding sites in the β1 and β3 A domains and their coordinating residues. Cation-binding site mutants made in β1 are shown in bold. Mutations were based on β3 structure as identified in the aVβ3 crystal structures. The cation-coordinating residues in β3 are shown alongside the corresponding residues in β1. Coordinating residues found only in the unliganded structure are marked with ‘*’ (12) and those found only in the liganded structure are marked with ‘**’ (13).

Cation binding site	Residues
MIDAS	β3	Asp119	Ser121	Ser123	Glu220	Asp251
	β1	Asp130	Ser132	Ser134	Glu229	Asp259
	mutation	D130A
ADMIDAS	β3	Ser123	Asp126	Asp127	Met335*	Asp251**
	β1	Ser134	Asp137	Asp138	Ala341	Asp259
	mutation		D137A	D138A
LIMBS	β3	Asp158	Asn215	Asp217	Pro219	Glu220
	β1	Glu169	Asn224	Asp226	Pro228	Glu229
	mutation	E169A E169D		D226A