Table III.

Apparent affinities of collagen I for α2β1-Fc integrins. Binding measurements were made in the presence of 1 mM Mn²⁺. Apparent affinity (App. K_d) values were estimated by non-linear regression analysis. Data shown are the mean ± S.D. from three experiments for each recombinant integrin.

	App. Kd (nM)	S.D. (nM)
FLα2β1	0.92	0.16
TRα2β1	1.52	0.23
α2[I332G] β1	1.07	0.06
α2[I332G]] β1[D130A]	9.92	1.32
α2[I332G] β1[D137A]	8.17	2.14
α2[I332G] β1[D138A]	2.94	0.29
α2[I332G] β1[D226A]	6.22	1.25
α2[I332G] β 1[E169A]	4.87	0.69
α2[I332G] β1 [E169D]	1.17	0.06