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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Biochem J. 2009 Nov 11;424(2):179–189. doi: 10.1042/BJ20090992

Figure 1. Preparation and characterization of recombinant soluble α5 integrins.

Figure 1

(A) Western blotting of recombinant-soluble zebrafish (zf) α5β1-Fc integrins. The expected migration positions of α,β heterodimer and α,α homodimers are indicated by arrows. In each case a predominant band at ~ 240 kDa is observed, at the expected size for the zfα5β1-Fc heterodimer. (B) Western blotting of recombinant-soluble human (hu) α5β1-Fc integrins. A predominant band at ~ 260 kDa is observed, at the expected size for the huα5β1-Fc heterodimer. These bands are not observed in proteins purified from the supernatants of mock-transfected cells.