Effect of JSM6427 (A) and CRRETAWAC (B) on binding of zfα5(SGAA)β1-1 swap zfα5(RGAA)β1-1 swap, zfα5(RGRA)β1-1 swap, zfα5(SWAA)β1-1 loop swap and wild-type zfα5β1-1 to 70K fragment of zebrafish fibronectin. One experiment, representative of three separate experiments. In this set of experiments, apparent KI values for JSM6427 were calculated as 25 ± 4, 29 ± 6, and 4.2 ± 0.2 nM (mean ± SD) for zfα5(SGAA)β1-1, zfα5(RGAA)β1-1, and zfα5(SWAA)β1-1, respectively.