Human fibroblasts were fixed and stained using indirect immunofluorescence for: a) α5 integrin [SNAKA51 (left panels) or SNAKA52 (center panels) or mAb11 (right panels), green], fibronectin (blue) and αV integrin as a focal adhesion marker (L230, red); or b) Top panel β1 integrin in a primed conformation (9EG7, red) and α5 integrin (SNAKA51, green), middle panel total β1 integrin (mAb11 red) and α5 integrin (SNAKA51, green), bottom panel β1 integrin in a primed conformation (9EG7, red) and total β1 integrin (mAb11 green). Bar 20 μm, insert bar 5μm.