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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: J Cell Sci. 2004 Dec 22;118(Pt 2):291–300. doi: 10.1242/jcs.01623

Figure 4. The SNAKA51 epitope maps to the calf domains of the α5 subunit.

Figure 4

a) Diagrammatic representation of the extracellular domains of the α5 and β1 integrin subunits indicating the position of truncations for the various recombinant constructs used in this study. b) The SNAKA51 binding site on the α5 integrin subunit was mapped using a sandwich ELISA of Fc-tagged truncated or individual domains of recombinant integrin. The Fc-tagged protein was specifically captured using anti-Fc antibodies, and then binding of SNAKA51 to the integrin was measured using enzyme-conjugated anti-mouse secondary antibody.