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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: J Cell Sci. 2004 Dec 22;118(Pt 2):291–300. doi: 10.1242/jcs.01623

Figure 7. SNAKA51 promotes fibronectin incorporation into the deoxycholate-insoluble matrix fraction.

Figure 7

Human salivary gland cells (HSG) were treated overnight with a) a range of concentrations of biotinylated fibronectin, or biotinylated fibronectin (10 μg/ml) and b) harvested at different time points, or c) with anti-integrin antibodies (25 μg/ml), or d) a range of concentrations of anti-α5 (SNAKA51). The cells were extracted with deoxycholate buffer, and the insoluble matrix fraction was collected and Western-blotted. Upper panel indicates biotinylated fibronectin incorporation into the insoluble matrix fraction. Lower panel indicates cytokeratin as internal loading controls for the HSG cells.