Figure 1.

Production of iPS cell-derived neurons follows basic developmental steps. (a) Keratinocytes are reprogrammed into iPS cells by forced overexpression of the Yamanaka factors (OSKM). After differentiation into ectodermal cells neural stem cells can be isolated from the centers of the forming neural rosettes. These neural stem cells can be subsequently differentiated into neurons, (b) proliferating keratinocyte culture, (c) iPS cell colony in a feeder-free culture, (d) neural rosette shortly before dissection and isolation of neural stem cells, (e) adherent culture of neural stem cells, and (f) iPS cell-derived neurons after four weeks of culture.