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. 2012 Mar 18;2012:629230. doi: 10.1155/2012/629230

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Copyright © 2012 Leonhard Linta et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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iPS cell-derived neurons express α-synuclein. (a) Immunofluorescence stainings of α-synuclein in TH (tyrosine hydroxylase) and TUBB3 (Tubulin beta-III) positive dopaminergic neurons after 5 months of differentiation show nuclear and vesicular localisation of α-synuclein, (b) and (c) RNA expression of α-synuclein (SNCA) and leucine-rich repeat kinase 2 (LRRK2) is upregulated in neurons compared to iPS cells and neural stem cells (NSCs) (normalized to the house-keeping gene HMBS), and (d) Subcellular fractionation of iPS cell-derived neurons shows nuclear (P1 fraction) and membrane associated, likely synaptic localization (P2 fraction) of α-synuclein.