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Effect of F094 or avicins on release of cytochrome c from mitochondria. Jurkat cells (1 × 107) were treated with F094, avicin D, or avicin G (all 2 μg/ml) at 37°C for the indicated time periods. Cells were homogenized, and lysates were assayed for cytochrome c levels by Western blot analysis as described in Methods.
