Figure 4.

(A) Kinetics of cytochrome c release from the mitochondrial fraction of Jurkat cells in a cell-free system. Mitochondria were isolated as described in Methods. Avicin G (2 μg/ml) treatment was given for 0, 1, 2, 5, 10, and 20 min at 37°C. (B) Dose–response of avicin G-induced cytochrome c release. The isolated mitochondria were treated with different concentrations of avicin G for 10 min at 37°C. (C) Effect of caspase inhibitors on avicin G-induced cytochrome c release. The isolated mitochondria were pretreated with DEVD-CH₂F (25 μM) or zVAD-fmk (25 μM) for 5 min at 37°C. This was followed by treatment with avicin G (2 μg/ml) for 10 min at 37°C. Release of cytochrome c was analyzed by Western blot as described in Methods.