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. 2001 May 8;98(10):5821–5826. doi: 10.1073/pnas.101619098

Figure 5.

Figure 5

(A) Kinetics of caspase-3 activation induced by F094 or avicins. Jurkat cells (1 × 106) were treated with 2 μg/ml of F094, avicin D, or avicin G for different time periods. Caspase-3 activity in the cytosolic extracts of these cells was determined as described in Methods. (B) Cleavage of PARP by F094 or avicins. Jurkat cells (3 × 106) were treated with 2 μg/ml of the agents for the indicated time periods. Cell lysates were prepared and assayed for cleavage of PARP as described in Methods. (C) Effect of zVAD-fmk treatment on PARP cleavage induced by F094 or avicins. Cells (3 × 106) were cultured ± zVAD-fmk (100 μM) for 1 h at 37°C and then followed by treatment with 2 μg/ml of F094 or avicins for 4 h at 37°C. Cleavage of PARP was studied as described above.