Fig. 5.

CeA-BNST projection neurons express NR1 and/or μOR(A). In tissue processed for triple labeling, some areas of the neuropil are populated by dendritic profiles expressing NR1 and/or μOR, but not FG. Two dendritic profiles (NR1 ± μOR-d) show labeling for NR1 (thin arrows, long enhancement time) and μOR (thick arrows, short enhancement time). (B). Regions of the CeA processed for triple labeling show neuronal profiles singly labeled for NR1 or μOR, but not FG. A dendritic profile (NR1-d) showing labeling for NR1 (thin arrow, long enhancement time) is contacted by an unlabeled axon terminal. A small presynaptic structure (μOR-a) shows a single particle for μOR (thick arrow, short enhancement time). (C). An area of the CeA processed for triple labeling contains single and dual labeled neuronal profiles. A single labeled dendritic profile (μOR-d) containing a gold–silver particle (thick arrow, long enhancement time) and an NR1 and μOR labeled dendritic profile (NR1 ± μOR-d) are present in the neuropil, which also contains a FG labeled dendrite (FG-d). (D). Triple labeled dendritic profile from a CeA neuron (FG ± NR1 ± μOR-d). Large gold particles for NR1 (thin arrows, long enhancement time) are present near the plasma membrane and clustered intracellularly. A small gold particle for μOR (thick arrow, short enhancement time) is present intracellularly. This profile contains a multivesicular body (mvb) and is contacted by unlabeled axon terminals (ut). (E). Triple labeled dendritic profile of a CeA neuron (FG ± NR1 ± μOR-d). A large silver–gold particle for NR1 (thin arrow, long incubation time) is present near the plasma membrane. A small silver–gold particle for μOR (large arrow, short incubation time) is present intracellularly beneath the shaft of the neck of a spiny process (sp) that receives an asymmetric synapse (arrow head). Scale Bars: 0.5 µm.