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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Exp Neurol. 2011 Dec 29;234(1):112–126. doi: 10.1016/j.expneurol.2011.12.034

Fig. 6.

Fig. 6

Omission of primary antisera in triple labeling experiments(A). A soma from the CeA is co-labeled for NR1 and μOR (NR1 ± μOR-s). This profile was observed in a control experiment where tissue was processed for triple labeling of NR1, μOR, and FG with omission of the FG primary antisera (No-FG 1°). Despite the lack of immunoperoxidase labeling, large and small immunogold-silver particles for NR1 (thin arrows, long incubation time) and μOR (thick arrows, short incubation time) are visually distinguishable (inset). (B). A cell body from the CeA shows co-labeling for FG and μOR (FG ± μOR-s). This profile was observed when sampling from tissue processed for a control experiment, where sections were prepared for triple labeling of NR1, μOR, and FG with omission of the NR1 primary antisera (No-NR 1°). Diffuse immunoperoxidase FG labeling and large particles for μOR (thick arrows, long incubation time) are seen in this soma (inset). Scale Bar: 1 µm.

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