Figure 4.
Both valproic acid and TSA increase caspase 3/7 activity. (A) Cells were treated with valproic Acid (0.1–10 mM) or vehicle control for 12 or 24 h before the measurement of caspase 3/7 activity. (B) Cells were treated with TSA (5–1000 nM) or vehicle control (DMSO) for 12 or 24 h before the measurement of caspase 3/7 activity. Caspase 3/7 activity was determined using Caspase-Glo 3/7 assay reagent (Promega) according to manufacturer’s protocol. Values represent the mean of luminescence signals of three independent experiments with standard deviations. *P<0.01